A critical challenge at core facilities are poor quality inputs such as FFPE, liquid biopsies, ancient DNA. These inputs are highly fragmented and degraded and perform poorly on standard NGS workflows. ClaretBio’s mission is to enhance the quality of sequencing data obtained from such challenging sample types. Our flagship library preparation method SRSLY generates high complexity libraries from picogram amounts of fragmented and degraded DNA. At this webinar, you will hear from two accomplished speakers who have developed robust workflows to challenging samples – non-plasma liquid biopsies and bones to generate high quality NGS data.
THE SERIOUS TALK - 7
In this presentation Dr. Troll discusses ClaretBio solution to NGS of high molecular weight DNA derived from FFPE samples. He covers the features of our DNA fragmentation reagent ForShear™
THE SERIOUS TALK - 6
In this presentation Dr. Katie Miller discusses the finding of the study conducted at Nationwide Children’s Hospital that pre-zygotic aneuploidy followed by post-zygotic partial reversion leads to a recurrent form of brain mosaicism-related epilepsy. Genomic analysis showed evidence of an extra, parentally-derived chromosome 1q in five of six patient brain tissues, which was not present in blood or buccal tissue – as evidenced by high-depth targeted sequencing of residual DNA using ClaretBio’s SRSLY
THE SERIOUS TALK - 5
The Missing in Action Recovery and Identification Project at the @uwmadison is a mission to retrieve personnel killed in overseas conflicts by performing excavations and research assistance to families of the missing. In the past this has been accomplished by combining military archives with eyewitness accounts and familial records to locate probable sites for excavation and recovery. Once potential identification elements are reclaimed, a portion can be sequenced to generate a putative identification. It is common that recovered elements are not the ideal source material for DNA extraction and subsequent sequencing library assembly due to combinations of elemental exposure and scarcity of materials. To overcome this obstacle, we have been treating these as ancient DNA samples that require modern DNA extraction and library assembly methodologies. Here I will report how I have employed these techniques to advance the Recovery and Identification missio
THE SERIOUS TALK - 4
In this video Dr. Lydia Freddolino talks about the how NGS tools such as SRSLY can be used for global profiling of RNA-DNA hybrids to reveal the role of R-loops in regulating bacterial genomic evolution.
The SERIOUS Talk-3
The SERIOUS Talk-2
In this webinar, Jordan Cheng (UCLA, School of Dentistry) describes the development of an extraction methods to capture highly degraded cell-free DNA from urine and plasma. A new class of cfDNA called ultra-short cell-free DNA are retained by this method and provides improved insights to the origin of cfDNA.
The SERIOUS Talk-1
In this first-talk of the webinar series, Ms. Adrienne Chang (Cornell University) talks about the challenges in metagenomic assays from cfDNA. She highlights utility of single-stranded library preparation such as SRSLY in retaining short fragments and improving overall quality of the data obtained from difficult to sequence DNA inputs
Genomic Social Hour #18: Ancient DNA | California Academy of Sciences
SRSLY. The Webinar
In this webinar we give an overview of the SRSLY technology including UMI addition, explain the SRSLY Kit catalog and introduce our new UMI demultiplexing software, and present data on its benefits for various input types, with an emphasis on cfDNA and RNA-Seq applications.